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HEBAU Team Found A Cluster of Glutathione S-Transferase Genes Provides Verticillium Wilt Resistance in Cotton

2019-06-06 00:00  


Prof. Ma Zhiying's Team Found A Cluster of Glutathione S-Transferase Genes Provides Verticillium Wilt Resistance in Cotton

近日,河北农业大学华北作物种质资源研究与利用教育部重点实验室马峙英教授团队完成的A newly-identified cluster of glutathione S-transferase genes provides Verticillium wilt resistance in cotton在植物学国际著名刊物The Plant Journal在线发表。

Recently, the team led by Prof. Ma Zhiying, working in the Key Lab for Research and Utilization of Crop Germplasm Resources in North China, Ministry of Education, finished a thesis titled A Newly-identified Cluster of Glutathione S-transferase Genes Provides Verticillium Wilt Resistance in Cotton”, which is online published on the worldwide famous journal in botany: “The Plant Journal”.


Through comparative genomic analysis of 11 species, the study confirmed that the upland cotton genome contains 9 classes of 123 Glutathione S-transferase Genes, and the type tau and MAPEG genes were latest formed, plus, the type tau had a huge expansion of the number of genes during the evolution of cotton. Analysis of 64 tau genes showed that a procotton originated 60 million years ago and a gene cluster of cocoa ancestor, which located on chromosome D09 of subgroup A underwent adaptive evolution and positive selection, in the process of the formation of Tetraploid upland cotton from Asian cotton and Raymond's cotton 1 to 2 million years ago. Transcriptome analysis showed that 17 GST genes induced by verticillium wilt were differentially expressed in resistant and susceptible varieties, including 14 tau genes, and 3 genes of the cluster were significantly up-regulated. Through the analysis of transgenic tobacco with the core gene GhGST of the cluster, it was found

that the resistance of transgenic plants to verticillium wilt was significantly improved, and the gene silencing test of upland cotton disease-resistant varieties proved that the cluster played an important role in fighting verticillium wilt, and further analysis found that the cluster was involved in the process of reactive oxygen homeostasis regulation of cells.


The results provide an important basis for the mechanism analysis of verticillium wilt resistance and disease resistance breeding of upland cotton. The research was funded by the national natural science foundation of China.

此外,马峙英教授团队2018年还在Nat GenetMol Plant PatholBMC Plant BiolTheor Appl GenetFront Plant SciPlant Cell Tiss Org等国际著名期刊,发表棉花产量、品质、抗逆性等重要性状的基因组研究和遗传改良相关论文,共8篇。论文发表题目及网址如下:

In addition, in 2018, Prof. Ma's team also published 8 papers on genome research and genetic improvement of important traits such as cotton yield, quality and stress resistance in Nat Genet, Mol Plant Pathol, BMC Plant Biol, Theor Appl Genet, Front Plant Sci, Plant Cell Tiss Org and other international famous journals. The title and website of the paper are as follows:

  1. Resequencing a core collection of upland cotton identifies genomic variation and loci influencing fiber quality and yield

  2. The cotton laccase gene GhLAC15enhanced Verticillium wilt resistance via increasing defense-induced lignification and lignin components in the cell wall of plants

  3. HyPRP1 performs a role in negatively regulating cotton resistance to V. dahliae via the thickening of cell walls and ROS accumulation

   4. A genome-wide association study uncovers novel genomic regions and candidate genes of yield-related traits in upland cotton

  5. Identification of SNPs and candidate genes associated with salt tolerance at the seedling stage in cotton (Gossypium hirsutum L.)

  6. Systematic analysis of cotton non-specific lipid transfer protein family revealed a special group that is involved in fiber elongation

  7. Molecular cloning of Ve promoters from Gossypium barbadense and G. hirsutum and functional analysis in Verticillium wilt resistance

                                         编辑:宣传部 姚运肖

                         Editor: Publicity Department Yao Yunxiao


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